Download free software for data analysis of MLPA products

Note: This page is preserved for historical reasons. Since the autumn of 2015 our use of RH-MLPA-Analysis.mdb has been replaced by Coffalyser.Net (www.mlpa.com). I.e we have thus stopped maintaining RH-MLPA-Analysis.mdb.

The software works especially smooth on MLPA fragment analysis data generated on an ABI 3100 system and sized to standard fragment lengths by GeneScan. Less smooth it handles data produced by GeneMapper or Peak Scanner software.

Examples in pdf-format of most of the test samples that are build into RH-MLPA-Analysis

The program "RH-MLPA-Analysis.mdb" analyses MLPA samples and prints a report per sample.
Input to the program is exported sizing table peak data produced by GensScan, GeneMapper or Peak Scanner software. The program requires Microsoft Access 2000 or higher.
Currently it handles these MRC MLPA probe sets: P021, P023, P026B, P033, P036B, P036D, P036-E1, P064B, P064-B2, P069, P069-A2, P095, P095-A2, P105-C1, P208-B1, P230-B1, P245, P245-A2, P264-A1, P277-A1, P286-A1, P291-A1. Examples to analyse are included for each of the mentioned probe sets.
See here for further details regarding download and installation of RH-MLPA-Analysis
   Latest version is 5.21 - 12 March 2010

The program "ABI-ExportTabularData" is for GeneScan users only and it exports the relevant data for each sample of a run.
Before using "ABI-ExportTabularData" you have to do an analysis of the peaks by the GeneScan program. GeneScan fits the peak sizes (in bp) to the ROX reference and updates the ABI sample files (".fsa" files) with these peak sizes. The exported sample data are placed in same folder as where the fsa-files are located (e.g. the actual run data folder on the ABI). Just like as if the user after analysis with the GeneScan program for each .fsa file of an size-caller-analysed run had selected "Export table" and then given the file of the exported tabular data the same name as the actual sample name.
See here for further details regarding download and installation of ABI-ExportTabularData
   Latest version is 19 March 2007.
   This version works better with Mac fsa files produced on e.g. an ABI 310 and converted to Windows.


Instructions:

Instructions for running the demo examples (pdf)
Detailed description of the analysis result sheets - exemplified by the P095-A2 kit (pdf)
Instructions for analysing GeneMapper data (pdf)
Instructions for analysing Peak Scanner data, see the demo samples instructions (pdf)
Instructions for analysing GeneScan data and to install/uninstall ABI-ExportTabularData (pdf)
Instructions for training RH-MLPA-Analysis for your own data (pdf)


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